Reporter

Part:BBa_K2805007

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


pGal1(Zif268op)x6-mRuby2

This is an mRuby red fluorescent protein driven by a mutated pGal1 promoter with 6 Zif268 zinc finger binding sites. This part is mean to be used in conjunction with BBa_K2805005 and BBa_K2805006. Zif268 will bind to the pGal1 promoter, activating transcription. When used with BBa_K2805005 and BBa_K2805006, the mRuby will report under red (650 nm wavelength) light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1608
    Illegal EcoRI site found at 411
    Illegal BglII site found at 891
    Illegal BamHI site found at 477
    Illegal XhoI site found at 885
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 411
    Illegal NgoMIV site found at 1928
    Illegal AgeI site found at 501
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None