Composite

Part:BBa_K2805010

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


{pPGK1-HY1}-{pTEF1-PcyA}

A heme oxygenase (HY1) and a bilin reductase (PcyA) for the production of the PCB and PΦB chromophores from tryptophan. These chromophores are used in PhyBNT, of the PhiReX system, and do not appear natively in S. cerevisiae. PhiReX will not work in yeast without these enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1740
    Illegal BglII site found at 885
    Illegal BglII site found at 3310
    Illegal BamHI site found at 1460
    Illegal XhoI site found at 1569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2863
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None