Coding

Part:BBa_K2805006

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


PIF3-NLS-VP16

This part codes for the helix-loop-helix protein PIF3 linked to an NLS and the VP16 transcriptional activator. This is piece of the PhiReX system which dimerizes with PhyBNT (BBa_K2805005) when PhyBNT is induced by red (650 nm wavelength) light. This PIF3 is fused to a transcriptional activator, so it will activate transcription of a downstream gene under the influence of red light and in the presence of PhyBNT-Zif268.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 1461
    Illegal PstI site found at 1921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1461
    Illegal PstI site found at 1921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 885
    Illegal BglII site found at 1400
    Illegal BamHI site found at 2484
    Illegal XhoI site found at 2727
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 1461
    Illegal PstI site found at 1921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 1461
    Illegal PstI site found at 1921
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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