Coding

Part:BBa_K2805003

Designed by: Meghan Bragdon, Cassandra Leach, Kevin Lorch, Linda Luo   Group: iGEM18_BostonU   (2018-10-09)


LOV2: NLS-VP16-EL222

This part codes for LOV2, a light-inducible transcription factor. It is made up of an NLS fused to VP16 and an EL222 region. When LOV2 is energized by blue (450-490 nm) light, it undergoes a conformational change that exposes a helix-turn-helix (HTH) DNA binding domain. The VP16 sequence of LOV2 also acts as a transcription factor, inducing the transcription of a downstream sequence. For example, an organism expressing both BBa_K2805002 and BBa_K2805003 will produce mRuby, a red fluorescent protein, when under a blue light. This sequence is optimized for use in S. cerevisiae.

Additionally, organisms expressing this plasmid should be kept in the dark. White light will also activate LOV2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1782
    Illegal BglII site found at 885
    Illegal XhoI site found at 1617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1187
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None