Coding

Part:BBa_K2805002

Designed by: Connor "Cass" Leach   Group: iGEM18_BostonU   (2018-10-09)


(C120op)x5-minCyc1-mRuby2-tCyc1

This part is an mRuby reporter backed by a C120op-based promoter site. This promoter is activated by LOV2, a light-inducible transcription factor made up of an NLS fused to VP16 and an EL222 region. An organism expressing both BBa_K2805002 and BBa_K2805003 will produce mRuby, a red fluorescent protein, when under a blue (450-490 nm) light.This sequence is optimized for use in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1086
    Illegal BglII site found at 369
    Illegal BamHI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1406
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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