Composite

Part:BBa_K3893026

Designed by: Stefania Soledad Montesinos Ludena   Group: iGEM21_Ecuador   (2021-10-15)


luxI constitutive expression

domestication.
Figure 1. SBOL Visual representation of the part


Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team Part Collection:

  • BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
  • BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
  • BBa_K2656019: Codon-optimized foe E. coli, luxI sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656019 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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