Part:BBa_K2656126

BMSTI transcriptional unit

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version.
• BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version
• BBa_K2656025: the BBa_J45004 BMSTI coding sequence in its Golden Braid standardized version
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 728
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]