Part:BBa_K2656025

BSMT1 Coding Sequence

Figure 1. DNA basic parts domestication. Third construction refers to CDS GB domestication.

Part BBa_K2656025 is the BMSTI coding sequence BBa_J45004 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. The protein encoded in this CDS catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a mint-like smell. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 664
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]