Part:BBa_K2656108

GFP_LVA transcriptional unit

Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design#Level1 Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

It is a composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com BioArt DNA toolkit palette: Printone ], as it can be used to express a green color with less intensity due to the degradation rate increased.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
• BBa_K2656009: the B0030 medium strength ribosome biding site in its Golden Braid compatible version
• BBa_K2656023: the BBa_K1399004 GFPmut3b_LVA sequence in its Golden Braid standardized version
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version.

The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.


Sequence and Features




Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]