Coding

Part:BBa_K2656023

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-22)

GFPmut3b_LVA Coding Sequence

domestication.
Figure 1. DNA basic parts domestication. Third construction refers to CDS GB domestication.

Part BBa_K2656013 is the GFPmut3b coding sequence with the LVA degradation tag. It is the BBa_K1399004 original sequence adapted to be Golden Gate compatible. Thus, it can be used with Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Braid] assembly methods. The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.

It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with a BsaI one-step reaction with the Golden Gate assembly protocol .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
None