Composite

Part:BBa_K2656101

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-22)

sfGFP transcriptional unit 2

Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part of our BioArt DNA toolkit palette: Printone , as it can be used to express a green bright color with medium intensity.


This transcriptional unit is composed of the following standardized parts from our Part Collection:

This transcriptional unit has been used to characterize the RBS basic part BBa_K2656009. Following this experimental protocol, we have obtained the parameters to valide our constitutive model and the results of the next graphs have been obtained after an optimization and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.

RBS experiment.
Figure 1. RBS expression experiment with K2656008, K2656009 and K2656012 basic pieces
RBS experiment.
Figure 2. RBS expression experiment with K2656008 and K2656012 basic pieces
Table 1. Optimized parameters for the BBa_ K2656101.
Parameter Value
Translation rate p p = 0.2059 min-1
Dilution rate μ μ = 0.01492 min-1
PoI degradation rate RBS Experiment 2: dp = 0.00818 min-1

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None