Part:BBa_K2656018

amilCP Coding Sequence

Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656018 is the amil Chromoprotein coding sequence BBa_K592009 compatible with both BioBricks and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

The characterization of this protein (and by extension of all the other part that codify for the amilCP) was performed with our transcriptional unit BBa_K2656113. This transcriptional unit was assembled in a Golden Braid alpha 1 plasmid including the following parts:

• BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
• BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
• BBa_K2656018: This part.
• BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]

In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we obtained the absorbance spectrum in the conditions of our equipment. By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#spectra protocol] with the parameters of Table 1, Figure 1 was obtained.

Parameter	Value
Number of samples	3
Wavelength measurement range (nm)	[300-700]
Table 1. Parameters used to obtain the spectum

Figure 2. amilCP absorbance spectrum

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]