Composite

Part:BBa_K200019

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)

pLacI+RBS+RFP+TT+pCstA+RBS+GFP+TT

This is the testing construct used by Imperial College's 2009 iGEM team to assess the two inducible promoters pLacI and pCstA through the expression of reporter molecules RFP and GFP in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.

This BioBrick comprises a ligation of the registry parts for the promoter LacI+PL (BBa_R0011) and the RBS (BBa_B0034).

The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream translation.

The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression (more information on this part can be found on its registry page here).

The subparts used to make this composite are BBa_J5526 and BBa_K200018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1733


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Categories
Parameters
None