Part:BBa_K118011

PcstA (glucose-repressible promoter)

This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)

ShanghaiTech_China 2022's Characterization

In our team's project, giving E. coli the ability to detect and report carbon source starvation helped increase the robustness of the system. Therefore, we chose the PcstA promoter and characterized it fluorescently in DH5α bacteria. After experiments and later sequence improvement, the PcstA promoter can correspond well to carbon source starvation.
Information about the improved models of our invented parts can be found inBBa_K4115004BBa_K4115003BBa_K4115027

Figrure 1.The result of sfGFP (with LVA) expression under the conditions of different carbon source concentrations

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]