DNA Submission

(Redirected from Help:Submission)

All submissions must be sent directly from the team to the iGEM HQ address below, and not a third party (except for Genscript, for iGEM Teams in China). A valid tracking number must be provided.

Submission shipments must be declared and labeled as DNA (non-hazardous, non-regulated, non-infectious, for research purposes only). It is OK if your shipment gets stuck in customs, we will have your tracking number.

iGEM will not accept shipments that are mislabeled, disguised, or hidden to avoid customs. This may affect medal eligibility for your parts.

iGEM HQ will not accept any DNA at the Giant Jamboree. All submissions must ship from the team or lab's location.

Send a Part Sample to the Registry

You've designed new parts, added them to the Registry and are ready to send the sample DNA. You can start a new submission, or view a previous submission and track the progress as we receive it. Before starting, read the submission requirements and the submission kit instructions.

If you have any questions about part submissions, please contact hq (at) igem (dot) org.

Mailing Address

For all iGEM 2017 Teams and Labs

For 2017, iGEM Teams must ship their part samples by the deadline (October 27) with a tracking number. Teams must follow all shipping guidelines and requirements.

  • iGEM Foundation
  • Attn: Vinoo Selvarajah
  • One Kendall Square
  • Suite B6104
  • Cambridge, MA 02139
  • USA
  • hq@igem.org
  • +1-617-500-3106
  • Mailing Address

    Only for iGEM 2017 Teams in China

    For 2017, iGEM HQ is collaborating with Genscript to help iGEM Teams in China submit their part samples. iGEM Teams in China will ship their part samples to Genscript by the ship by deadline (October 27) with a tracking number, and Genscript will forward these shipments to iGEM HQ. Teams must still follow all shipping guidelines and requirements.

  • Ship to address: 江苏省南京市江宁区江宁科学园雍熙路28号 南京金斯瑞生物科技
  • Receiver: 张楚麟 Zhangchulin
  • Tel: 400-025-8686转分机号5837 / 13218091753
  • Email: zhangchulin@genscript.com
  • more info...


  • DNA Submission


    Requirements

    Here's how part samples are sent to the Registry

    Why pSB1C3?

    Why do samples need to be shipped in pSB1C3?

    Detailed Instructions

    Instructions on how to fill out the Registry's submission form.

    Submission Kit

    Registry groups are provided with a submission kit to ship their part samples.


    Have questions on requirements or shipping part samples? Check the FAQ or contact us at hq (at) igem . org .

    Submission Requirements

    You've added your part to the Registry, but before you send a DNA sample to the Registry, here are the Registry's requirements for documenting parts and preparing part samples for submission. If you do not follow these requirements your submission will not be accepted.

    If you have questions or issues concerning the requirements, contact hq (at) igem (dot) org. Please contact us early!



    FAQ: Submission Requirements

    What are the requirements for submitting a part sample to the Registry?

    The requirements for submission...

    Please see the Registry's submission requirements page for more information.

    Why do parts sent to the Registry need to be BioBrick RFC10 compatible

    iGEM and the Registry are built on standard parts which is vital to making biology easier (and more predictable) to engineer, for high school teams & university teams to research labs. It is also why iGEM has made BioBrick RFC10 compatibility a requirement; the parts that we receive from teams and labs can be used and assembled by everyone easily and reliably, without the need to substantially alter, experiment, or research alternate methods.

    BioBrick RFC10 compatibility ensures that...

    • users can assemble these parts with 3A Assembly to build new devices and projects
    • part samples can be easily moved from one plasmid backbone to another (for shipping, measurement, operation etc.)
    • the Registry can test and maintain all part samples it receives in the same way


    Does the Registry only allow BioBrick RFC10 compatible parts?

    If you are submitting a physical sample of a part to the Registry, that part must be BioBrick RFC10 compatible. iGEM teams are required to send part samples to the Registry to fulfill medal requirements and be eligible for awards. These parts must be BioBrick RFC10 compatible

    The parts you add and document on the Registry do not need to be BioBrick RFC10 compatible. The Registry supports multiple assembly standards and is also open to integrating new ones. You can easily see what Registry supported assembly standards your part is compatible with, by taking a look at the Sequence & Features box.


    My part is not BioBrick compatible

    Before working with your part in the lab (characterizing/measuring), make sure it is BioBrick compatible. If not...

    • Synthesize your part to remove any illegal restriction sites, through synonymous substitutions (silent mutations).
    • Use site-specific mutagenesis to remove any illegal restriction sites, through synonymous substitutions (silent mutations).

    Currently, the Registry only accepts parts samples that are BioBrick compatible. If you have questions about this requirement, please don't hesitate to contact iGEM HQ (hq (at) igem . org)! Contact us early and we may be able to address your concerns.


    Why do my parts need to be submitted in pSB1C3

    Having a shipping standard backbone lets the Registry receive and handle all parts in the same way: using the same growth conditions and quality control measures. With over 1000 new parts submitted each year, this improves our ability to handle, maintain, and test new parts. When anyone uses the part, they'll know exactly what to expect. They can easily unpackage it from the shipping plasmid backbone, and transfer it into a different backbone for a specific use.

    Shipping in pSB1C3 ensures...

    • Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
    • users can assemble these parts with 3A Assembly to build new devices and projects
    • The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
    • pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts
    • part samples can be easily moved from one plasmid backbone to another (for shipping, measurement, operation etc.)


    My part cannot be submitted in pSB1C3 because of ______

    First, contact iGEM HQ (hq (at) igem . org)! Contact us early and we may be able to address your concerns.

    There may be a reason why a part sample cannot be submitted in pSB1C3. For example...

    • The part contains chloramphenicol resistance. Contact iGEM HQ first. You must use pSB1K3 instead.
    • The part is toxic to cells in a high copy plasmid backbone. Contact iGEM HQ first. You must use pSB3C5 instead.


    My part is not meant to be used/operated in pSB1C3, can I use ______ instead

    Unfortunately, no. Please remember, that the shipping standard backbone lets the Registry receive and handle all parts in the same way. In turn, when these samples are distributed to users, they'll know exactly what to expect. They can easily unpackage it from the shipping plasmid backbone, and transfer it into a different backbone for a specific use.


    We've created a new plasmid backbone. How can we submit it to the Registry?

    While the Registry does not specialize in plasmids, you can send your plasmid backbone with BBa_J04450, the Registry's default insert. Your plasmid backbone must be able to replicate in E. coli.


    Can we send part samples after the deadline?

    If you're an iGEM Lab, you can send part samples at anytime.

    If you're an iGEM Team, your part samples must arrive on or before the deadline on the Calendar, in order to be evaluated for the competition. Teams can continue to submit part samples after the deadline, however these parts will not be evaluated by the iGEM judges and are not eligible for medal criteria or awards.

    For iGEM 2017, the deadline reflects a send by date of October 27th. Parts must be sent out on or before October 27th. Shipments must have a tracking number, as that will let us know when parts shipped, and when to expect arrival.