Help:Plasmid Backbones

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Plasmid Backbone Overview

    The Registry divides a plasmid into two separate components:
  • the part
  • the plasmid backbone

Why would you want to use more than one plasmid backbone?

One part can be used in several different plasmids backbones. It's easy to move a part from one plasmid backbone to another.

Assembly

    Use any assembly method you want!
  • You may have a plasmid backbone that works well with your assembly method (and standard)
  • Just make sure that your parts are BioBrick compatible when it's time to ship them in pSB1C3 to the Registry

Operation

    Operate your part in the plasmid backbone that works for your system
  • Maybe your part uses a lot of cellular resources, so you'll want to operate it in a low copy plasmid backbone
  • Or you want to test your part in different organisms by using a multi-host plasmid backbone

Measurement

    Use a plasmid backbone to measure your part
  • You may want to use a plasmid backbone that will help you measure attributes of your part
  • Have a library of promoters you want to test? Use a plasmid backbone with a built in coding region.
  • Don't forget, documenting and characterizing your part is important!

Shipping

    After you're done assembling, operating, and measuring your part, ship it to the Registry in pSB1C3
  • Shipping in pSB1C3 lets the Registry maintain and test all parts in the same way
  • Anyone who wants to use your part can easily and reliably move it into another plasmid backbone

Prefix and Suffix

    The Prefix and Suffix flank the part on either side:
  • the part does not include the prefix and suffix
  • the plasmid backbone is defined as the sequence starting with the suffix and ending at the prefix


Learn More About:

Plasmid Backbones: Overview | Shipping in pSB1C3 | Features | Linearized Plasmid Backbones | Plasmid Backbone Catalog