Primer
VR
Part:BBa_G00101
Designed by: Tom Knight Group: Antiquity (2004-05-25)
Reverse primer for sequencing/amplifying BioBrick parts (VR)
Originally named VR, This reverse primer binds downstream of the standard BBa_MSC on biobrick vectors. Used for general-purpose PCR and sequencing of Biobrick parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//primer/part/amplification
//primer/part/sequencing
//primer/part/sequencing
| direction | R |
| len_end | 175 |
| len_fr | 314 |
| len_start | n/a |
| pct_gc | 50 |
| target | BioBrick plasmid backbones |
| t_m | 51.1 |