Well Documented Parts

At the beginning of iGEM, it was difficult to get experiments to work as expected. With not much working in the lab, little documentation was produced. As the competition matured and labs became more used to making and testing BioBricks, more experiments worked, and documentation began to improve. We then changed the rules, requiring iGEMers to submit highly-documented parts to be eligible for medals. Now, iGEMers submit amazing parts with abundant documentation, not because we require them, but because iGEMers want to. They know this is how to get future teams to use parts and is a critical part of enriching the synthetic biology community.

The job of every iGEMer and contributor to the Registry is to make their part as awesome as possible to inspire users to use it in following years. We encourage everybody who contributes to the Registry to complete as much information as possible and to perform as much characterization as possible before sending us a sample. We also encourage teams to add parameters and categories to their parts. We use these metrics to create part tables that are automatically updated when you add categories.

We're compiled several "Top 10" lists of the most documented parts in the Registry. Please note that to calculate the amount of documentation, we look at the HTML code, which sometimes picks up a lot of code that isn't displayed. We also count information on the Design and Experiences pages, but don't take images into account at this time. Although there may be heuristic errors in our calculations, we're working on making them better and more accurate. Have a look at the parts below to see the most documented examples in the Registry.

Top 10 Most Documented Parts

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NameDescriptionLengthCreated byDocumentaionTypeStatus
BBa_K404003[AAV2]-Rep-VP123(ViralBrick-587KO-empty)_p5-TATAless4386Freiburg Bioware 201062110ProjectIt's complicated
BBa_K404002[AAV2]-Rep-VP123_p5-TATAless4386Freiburg Bioware 201047172ProjectIt's complicated
BBa_K863005ecol laccase from E. coli with T7 promoter, RBS and His-tag1605Isabel Huber42988CodingIn stock
BBa_K404122[AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR 3659 Freiburg Bioware 201042792ProjectIt's complicated
BBa_K863000bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag1587Isabel Huber41945CodingIn stock
BBa_K863006ecol laccase from E. coli1551Isabel Huber41372CodingIn stock
BBa_K863001bpul laccase from Bacillus pumilus1533Isabel Huber39446CodingIn stock
BBa_K404163pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Tag) 2830Freiburg Bioware 201038331ProjectIn stock
BBa_K741011plac-anticro-T-pCon(0.244)-RBS-crogfp-T1685Wuyang Chen37530CompositeIt's complicated
BBa_K1172501Outer membrane porin OprF from Pseudomonas fluorescens1298Lukas Rositzka35063CodingIt's complicated

Top 10 Most Documented Promoters

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NameDescriptionLengthCreated byDocumentaionTypeStatus
BBa_J61051[Psal1]1268John Anderson16804RegulatoryIn stock
BBa_K678001PalcA, inducible Aspergillus nidulans promoter843DTU-Denmark-213955RegulatoryIn stock
BBa_K754000S. elongatus PCC7942 psbAI promoter246iGEM Valencia 201213052RegulatoryIt's complicated
BBa_K1216007Variant of the wild-type pLuxR promoter with lower sensitivity55Meret Arter11819RegulatoryIn stock
BBa_K678000DMKP-P6, Aspergillus nidulans promoter548DTU-Denmark-211125RegulatoryIn stock
BBa_K774002Comparator Circuit Part 1130NRP-UEA-Norwich10859RegulatoryIt's complicated
BBa_K774003Comparator Circuit Part 2130NRP-UEA-Norwich10850RegulatoryIt's complicated
BBa_K801020KlADH4 yeast promoter, ethanol inducible952Simon Heinze9706RegulatoryIt's complicated
BBa_K774000Bacterial-Mammalian Promoter: BBaK216005 + BHN + CArG promoter sequence E9-ns2208NRP-UEA-Norwich8621RegulatoryIn stock
BBa_K625003Pu promoter short version203Michael Eichenberger8557RegulatoryIt's complicated

Top 10 Most Documented Coding Regions

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NameDescriptionLengthCreated byDocumentaionTypeStatus
BBa_K863005ecol laccase from E. coli with T7 promoter, RBS and His-tag1605Isabel Huber42988CodingIn stock
BBa_K863000bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag1587Isabel Huber41945CodingIn stock
BBa_K863006ecol laccase from E. coli1551Isabel Huber41372CodingIn stock
BBa_K863001bpul laccase from Bacillus pumilus1533Isabel Huber39446CodingIn stock
BBa_K1172501Outer membrane porin OprF from Pseudomonas fluorescens1298Lukas Rositzka35063CodingIt's complicated
BBa_K525710DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag2064Anna Drong34812CodingIn stock
BBa_K1172201GldA - glycerol dehydrogenase from Escherichia coli1104Lukas Rositzka26932CodingIn stock
BBa_K863022bhal laccase from Bacillus halodurans with constitutive promoter J23100, RBS and HIS tag1586Isabel Huber25159CodingNot in stock
BBa_K863021bhal laccase from Bacillus halodurans 1503Isabel Huber25095CodingIn stock
BBa_K525515Fusion protein of BisdA and BisdB1602Anna Drong23909CodingIn stock

Top 10 Most Documented Ribosome Binding Sites

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NameDescriptionLengthCreated byDocumentaionTypeStatus
BBa_K1100014ALeaderT (with insulator)106Hao-tian Guo, Chenxi He10799RBSIn stock
BBa_K1100001ALeaderT:The First Artificial Triphase Riboswitch87Hao-tian Guo8736RBSNot in stock
BBa_K1100000ALeader75Hao-tian Guo5649RBSIt's complicated
BBa_K1017202Regulation RBS-221Wen-Hui Cheng5345RBSIn stock
BBa_K1084103SD633Ami Kawahata2793RBSIn stock
BBa_K1084104SD833Ami Kawahata2793RBSIn stock
BBa_K1084102SD4 33Ami Kawahata2793RBSIt's complicated
BBa_K1084101SD233Ryotaro Nouda2789RBSIn stock
BBa_K3900025'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 680349Cody Tramp2411RBSIt's complicated
BBa_K1100076RNA-IN S4934Hao-tian Guo2166RBSNot in stock

Top 10 Most Documented Terminators

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NameDescriptionLengthCreated byDocumentaionTypeStatus
BBa_K404108hGH terminator481Freiburg Bioware 20107779TerminatorIt's complicated
BBa_K731721T7 terminator48Giacomo Giacomelli, Anna Depetris7386TerminatorIn stock
BBa_K731722T1 terminator from E. coli rrnB79Anna Depetris, Giacomo Giacomelli6866TerminatorIn stock
BBa_K801012ADH1 yeast terminator349Georg Schützinger4652TerminatorIn stock
BBa_K1023002xlnD terminator836Michiel Karrenbelt2074TerminatorIn stock
BBa_B0054Transcriptional terminator69Reshma Shetty1731TerminatorNot in stock
BBa_K1114301This is MoClo formatted part of BBa_B0015137Devina Desai1676TerminatorIn stock
BBa_K1114302This is MoClo formatted part of BBa_B001137Devina Desai1676TerminatorIn stock
BBa_K1114300This is the MoClo formatted part of BBa_B0015.137Devina Desai1676TerminatorIn stock
BBa_K165018SV40 nuclear localization sequence + ADH1 transcription terminator253John Szymanski1664TerminatorIt's complicated