Part:BBa_I14017:Experience
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Applications of BBa_I14017
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ETH Zurich 2014 |
Characterization of two-order crosstalk on the promoterBackground informationIdeally, C4-HSL (inducer) binds to RhlR (receptor) and this complex then activates the transcription of the gene regulated by Prhl. However, when using different inducers (3OC6-HSL and 3OC12-HSL) and receptors (LuxR and LasR), they can cross react to form complexes which activate the transcription of the gene regulated by Prhl. The effect of these possible combinations on the promoter have been characterized in detail below. First-order crosstalkFirst Level crosstalk: RhlR binds to different HSL and activates the promoterSecond Level crosstalk: other regulatory proteins, like LuxR, bind to their natural HSL substrate and activates the promoterSecond order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LuxR, bind to different HSL and activates the promoter. Results
Modeling crosstalkEach experimental data set was fitted to an Hill function using the Least Absolute Residual method. The fitting of the graphs was performed using the following equation :
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Antiquity |
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