Part:BBa_C0179:Experience

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User Reviews

UNIQc8406308aeec67ab-partinfo-00000000-QINU

•••• ETH Zurich 2014

Characterization of two-order crosstalk Background information System considered First-order crosstalk First Level crosstalk: LasR binds to different HSL and activates the promoter Plas Second Level crosstalk: LasR binds to 3OC12-HSL, its natural HSL, and activates different promoter 400px Second order crosstalk: Combination of both cross-talk levels Results Table 1 Crosstalk matrix for the regulator LasR (BBa_C0179) In all the measurements conducted to create this matrix the regulator LasR was the basis and was induced in six different variations shown.

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

No review score entered. Northwestern 2011

The 2011 Northwestern iGEM team used this part as a part of our Pseudomonas Aeruginosa biosensor. We were able to successfully express RhlR (C0171) continuously in our system. BBa K575032

••••• iGEM Dundee 2014

Dundee iGEM 2014 used this lasR coding sequence to build two composite parts termed BBa_K1315009 and BBa_K1315010. These were designed as biosensors for Pseudomonas aeruginosa AutoInducer-1 (PAI-1), and were to be used in a bio-electronic device to improve diagnostics for Cystic Fibrosis patients. Details of experimental work are logged on the experience pages of BBa_K1315009 and BBa_K1315010.