Device

Part:BBa_K323089:Design

Designed by: Tina Lebar   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 18:24, 26 October 2010 by TinaL (Talk | contribs) (→‎Source)

In vivo testing device for protein-DNA binding: part 2 (lacZ_DTER_pBAD_BsaI_DTER)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4216
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4496
    Illegal BsaI.rc site found at 4472
    Illegal SapI site found at 4198


Design Notes

Part:BBa_I732019 was ligated into a vector containing Part:BBa_B0015 as a front insert. Part:BBa_K323110 was then ligated with the resulting construct as a back insert.

=Source

Part:BBa_B0015 and Part:BBa_I732019 were taken from the Registry of parts, and Part:BBa_K323110 was previously constructed by our team (for more information see Part:BBa_K323110:Design)

References