Device
Clone in

Part:BBa_K323110

Designed by: Tina Lebar   Group: iGEM10_Slovenia   (2010-10-20)

pBAD_BsaI_DTER

This part is composited from the pBAD promoter (Part:BBa_I0500), a double terminator (Part:BBa_B0015) and a BsaI restriction site inbetween. The BsaI restriction endonuclease cuts the DNA outside of its recognition site. BsaI restriction site (clone in site) is designed in such a way that any BioBrick part, cut with XbaI and NotI enzymes can be ligated into this vector cut with the BsaI enzyme.

Figure: BsaI restriction site. The BsaI restriction endonuclease cuts the DNA outside of its recognition site. The clone in site was designed in such a way, that any BioBrick gene, cut with XbaI and NotI enzymes could be ligated into the vector, cut with the BsaI enzyme.

Therefore, the purpose of this part is cloning any BioBrick protein coding sequence between a promoter and a terminator in only one ligation step instead of two.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1260
    Illegal BsaI.rc site found at 1236
    Illegal SapI site found at 962


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Categories
Parameters
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