Part:BBa_K323089:Design

In vivo testing device for protein-DNA binding: part 2 (lacZ_DTER_pBAD_BsaI_DTER)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4441
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4381
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4216
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4496
  Illegal BsaI.rc site found at 4472
  Illegal SapI site found at 4198
  

Design Notes

Part:BBa_I732019 was ligated into a vector containing Part:BBa_B0015 as a front insert. Part:BBa_K323110 was then ligated with the resulting construct as a back insert.

Source

Part:BBa_B0015 and Part:BBa_I732019 were taken from the Registry of parts, and Part:BBa_K323110 was previously constructed by our team (for more information see Part:BBa_K323110:Design)