Device
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Part:BBa_K323110:Design

Designed by: Tina Lebar   Group: iGEM10_Slovenia   (2010-10-20)

pBAD_BsaI_DTER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1260
    Illegal BsaI.rc site found at 1236
    Illegal SapI site found at 962


Design Notes

Primers were designed for the BsaI clone in site, and after primer annealing the resulting fragment was ligated into the vector containing the pBAD promoter (Part:BBa_I0500) as a back insert. The construct was then ligated into the vector containg DTER (Part:BBa_B0015) as a front insert.

Source

The pBAD promoter (Part:BBa_I0500) and double terminator (Part:BBa_B0015) were taken from the Registry of parts, and primers for the BsaI clone in site were designed and ordered from Sigma-Aldrich.

References