Generator

Part:BBa_K325219

Designed by: Bill Collins and Emily Knott   Group: iGEM10_Cambridge   (2010-09-15)
Revision as of 11:33, 24 September 2010 by PM (Talk | contribs)

Input: L-Arabinose
Output: Light

pBad/araC
I0500
Luciferase/LRE
K325210
Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        Add Data       


Description
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.


Performance

Experiment1 Characteristic1 Value1
Transfer Function Maximum Output 6.6 PoPS cell-1
Hill coefficient 1.6
Switch Point 1.5E-9 M 3OC6HSL, exogenous
Response time: <1 min
Input compatibility Strong response to 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
Weak response to C4HSL, C10HSL, C12HSL
Stability Genetic Stability
(Low/High Input)
>92/>56 generations
Performance Stability
(Low/High Input)
>92/>56 generations
Demand Internal Demand
(Low/High Input)
Not measured
Transcriptional output demand:
(Low/High Input)
Nt = length of downstream transcript in nucleotides
(0/6xNt) nucleotides cell-1 s-1
(0/1.5E-1xNt) RNAP cell-1
Growth Rate
(Low/High Input)
54/59 min Doubling time

1Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.

References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61,6-17.

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Categories
Parameters
None