Part:BBa_K3893026

luxI constitutive expression

Figure 1. SBOL Visual representation of the part

Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part [1] to assemble the release system device ([2]) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, fundamental piece of our population oscilator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
• BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
• BBa_K2656019: the K2656013 luxI sequence in its Golden Braid standardized version
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon optimized for E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]