Composite
Part:BBa_K2934004
Designed by: Nir Litver Group: iGEM19_Technion-Israel (2019-10-10)
AmyE - Glucose Oxidase-Histag for Bacillus subtilis
This part contains the Glucose Oxidase (GOx) gene (BBa_K2934000), connected to the secretory signal peptide AmyE (from the alpha-amylase gene) (BBa_K2273023), using a short nine amino-acids linker (BBa_K2934005).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 156
Illegal BsaI site found at 487
Illegal BsaI.rc site found at 433
Illegal BsaI.rc site found at 1258
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Categories
Parameters
None |