Composite

Part:BBa_K2656109

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-22)
Revision as of 22:29, 14 October 2018 by Carolinarp (Talk | contribs)


mRFP1 transcriptional unit 1

Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
  • BBa_K2656009: the B0030 medium strength ribosome biding site in its Golden Braid compatible version
  • BBa_K2656014: the BBa_E1010 mRFP1 sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

    • Thus, mRFP1 sequence is assembled with a very low strength promoter and relative strong RBS.

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 11
      Illegal NheI site found at 34
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 620
      Illegal AgeI site found at 732
    • 1000
      COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None