1 Registry Star
Project
WOBS
Part:BBa_J45700:Experience
Designed by: MIT iGEM 2006 Group: iGEM06_MIT (2006-10-30)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45700
User Reviews
UNIQb401ba9116de2ca3-partinfo-00000000-QINU
|
••• |
The wintergreen odor biosynthetic system BBa_J45700 appears to produce some methyl salicylate from the cellular metabolite chorismate. |
UNIQb401ba9116de2ca3-partinfo-00000003-QINU
Characterization
Gas chromatography analysis of wintergreen odor biosynthetic system
Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.