Project
WOBS

Part:BBa_J45700:Experience

Designed by: MIT iGEM 2006   Group: iGEM06_MIT   (2006-10-30)

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Please enter how you used this part and how it worked out.

Applications of BBa_J45700

Production of methyl salicylate (wintergreen odor) from cellular metabolites.

User Reviews

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Reshma Shetty

The wintergreen odor biosynthetic system BBa_J45700 appears to produce some methyl salicylate from the cellular metabolite chorismate.

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Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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Characterization

Gas chromatography analysis of wintergreen odor biosynthetic system

Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.