Part:BBa_J45320

Salicylate generator

The salicylate generator (BBa_J45320) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45319). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

Usage and Biology

• BBa_J45320 produces an isochorismate pyruvate-lyase and an isochorismate synthase derived from the pchBA genes from Pseudomonas aeruginosa. PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Salicylate is the anion of salicylic acid and the two should be in rapid equilibrium. Salicylic acid is the precursor to methyl salicylate (wintergreen odor).
• See BBa_J45017 for details.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 389
  Illegal NgoMIV site found at 793
  Illegal NgoMIV site found at 918
  Illegal NgoMIV site found at 929
  Illegal NgoMIV site found at 1210
  Illegal NgoMIV site found at 1678
  
• 1000
  COMPATIBLE WITH RFC[1000]