Composite
Part:BBa_K784047:Design
Designed by: Asaf Blumental Group: iGEM12_Technion (2012-09-26)
Theophylline riboswitch + wt T7 RNAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 15
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The riboswitch had to fused to the RNAP using assembly PCR.
Source
Assembly PCR of BBa K784005 and BBa_I2032. BBa I2032 was received from Ann K. Ganesan.