Composite

Part:BBa_K784047:Design

Designed by: Asaf Blumental   Group: iGEM12_Technion   (2012-09-26)
Revision as of 08:42, 26 September 2012 by Registry (Talk | contribs)

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Theophylline riboswitch + wt T7 RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The riboswitch had to fused to the RNAP using assembly PCR.



Source

Assembly PCR of BBa K784005 and BBa_I2032. BBa I2032 was received from Ann K. Ganesan.

References