Composite
Part:BBa_K784047:Design
Designed by: Asaf Blumental Group: iGEM12_Technion (2012-09-26)
Theophylline riboswitch + wt T7 RNAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 15
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7 RNAP are adjacent to one another without any restriction site between them. There is an additional stop codon at the end of the gene.
Source
Assembly PCR of BBa K784005 and BBa_I2032. BBa I2032 was received from Ann K. Ganesan.