Part:BBa_K784005

Theophylline riboswitch 12.1

This part has been originally designed by Sean A. Lynch and Justin P. Gallivan. The part is a riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.
For this part to work properly, it must be directly fused to the starting codon of the downstream coding region.
This part is a duplicate of BBa_J89000 but with additional sequence notations and additional sequence upstream to the theophylline aptamer.
The physical DNA of this part hasn't been sent to the registry. However, a fusion product of this part with mCherry is available as BBa_K784006.

BOKU-Vienna 2019 - Improvement

The iGEM Team BOKU-Vienna improved BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way BBa_K3015004. The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Regsitry-Page of the described Composite Part BBa_K3015007

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 15
  
• 1000
  COMPATIBLE WITH RFC[1000]