Part:BBa_K325100

Input: L-Arabinose Output: Light

pBad/araC I0500 Luciferase/LRE K325210

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Description
This part generates a mutant of the luciferase from the North American firefly (L.cruciata) as well as this species' luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter (pBAD). D-Luciferin has to be added to obtain light output.

The part is codon optimised for expression in E.coli.

THIS PAGE IS CURRENTLY BEING UPDATED.

Performance

Experiment1	Characteristic1	Value1
Transfer Function	Maximum Output	6.6 PoPS cell-1
	Hill coefficient	1.6
	Switch Point	1.5E-9 M 3OC6HSL, exogenous
Response time:	<1 min
Input compatibility	Strong response to	3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
	Weak response to	C4HSL, C10HSL, C12HSL
Stability	Genetic Stability (Low/High Input)	>92/>56 generations
	Performance Stability (Low/High Input)	>92/>56 generations
Demand	Internal Demand (Low/High Input)	Not measured
	Transcriptional output demand: (Low/High Input) Nt = length of downstream transcript in nucleotides	(0/6xNt) nucleotides cell-1 s-1
		(0/1.5E-1xNt) RNAP cell-1
	Growth Rate (Low/High Input)	54/59 min Doubling time

¹Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.

[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.

[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.