Part:BBa_K2656013

sfGFP Coding Sequence

Part BBa_K2656013 is the super folder Green Fluorescent Protein coding secuence BBa_I746916 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid 3.0] assemply methods. It can be combined with other compatible part from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

The charcterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit BBa_K2656100. This transcriptional unit BBa_K2656100 was assembled in a Golden Braid alpha1 plasmid(REF) including the following parts:

• BBa_K2656004 BBa_K2656004: theJ23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
• BBa_K2656009 BBa_K2656009: theB0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
• BBa_K2656013 BBa_K2656013: This part.
• BBa_K2656026 BBa_K2656026: theB0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]

In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. To do this, we have used the transcriptional unit BBa_K2656100 assembled in a Golden Braid alpha1 plasmid(REF). By using this protocol (REF) with the parameters of Table 1, Figure 1 has been obtained.

Parameter	Value
Number of samples	6
Excitation Wavelength measurement range (nm)	[420-525]
Emission wavelenght (nm)	545
Emission Wavelength measurement range (nm)	[470-580]
Excitation wavelenght (nm)	450
Gain (G)	50
Table 1. Parameters used to obtain the spectra

Figure 1. sfGFP emission and excitation spectra

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 14