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Applications of BBa_C0171

User Reviews

UNIQe9dd6c88f6e80a2b-partinfo-00000000-QINU

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ETH Zurich 2014

Characterization of two-order crosstalk on the promoter

Background information

System considered

Modeling crosstalk

First-order crosstalk

First Level crosstalk: RhlR binds to different HSL and activates the promoter

Second Level crosstalk: other regulatory proteins, like LuxR, bind to their natural HSL substrate and activates the promoter

Second order crosstalk: Combination of both cross-talk levels

Results

**Table 1** Crosstalk matrix for the regulator LasR (BBa_C0171)
In all the measurements conducted to create this matrix the regulator RhlR was the basis and was induced in six different variations shown.

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

UNIQe9dd6c88f6e80a2b-partinfo-00000003-QINU

No review score entered. Northwestern 2011

The 2011 Northwestern iGEM team used this part as a part of our Pseudomonas Aeruginosa biosensor. We were able to successfully express LasR (C0171) continuously in our system. BBa K575024

Part:BBa_C0171:Experience