Generator
SG

Part:BBa_J45320

Designed by: André Green II   Group: iGEM06_MIT   (2006-10-20)
Revision as of 19:16, 8 March 2008 by Rshetty (Talk | contribs)

Salicylate generator

The salicylic acid generator (BBa_J45320) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45319). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

BBa J45320.png

Usage and Biology

  • BBa_J45320 produces an isochorismate pyruvate-lyase and an isochorismate synthase derived from the pchBA genes from Pseudomonas aeruginosa. PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Salicylic acid is the precursor to methyl salicylate (wintergreen odor).
  • See BBa_J45017 for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prct
//function/odor
s
Parameters
o_h
proteinPchA & PchB
rbs
tagNone