Part:BBa_J45017

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate

BBa_J45017 encodes an isochorismate pyruvate-lyase and an isochorismate synthase derived from the pchBA genes from Pseudomonas aeruginosa. PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Salicylic acid is the precursor to methyl salicylate (wintergreen odor).

Usage and Biology

pchB and pchA are at the downstream end of the pchDCBA operon that Pseudomonas species use to encode enzymes to make pyochelin, a molecule that helps them acquire iron. BBa_J45017 doesn't make a fusion protein but the coding regions of the two genes do overlap. The RBS for pchA is located at the end of the coding region of pchB.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 307
  Illegal NgoMIV site found at 711
  Illegal NgoMIV site found at 836
  Illegal NgoMIV site found at 847
  Illegal NgoMIV site found at 1128
  Illegal NgoMIV site found at 1596
  
• 1000
  COMPATIBLE WITH RFC[1000]