Difference between revisions of "Part:BBa K325909"
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'''References'''<br> | '''References'''<br> | ||
− | [http://www.jstor.org/stable/4449975 '''[1]:'''] J. Slock, (1995) Transformation Experiments | + | [http://www.jstor.org/stable/4449975 '''[1]:'''] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of ''Vibrio fischeri'',''The American Biology Teacher'', '''57''', 225-227. |
</div> | </div> | ||
− | [http://www. | + | [http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 '''[2]:'''] E.A. Meighen (1988) Enzymes and genes from the ''lux'' operons of bioluminescent bacteria, ''Annual Reviews in Microbiology'' '''42''', 151-176. |
− | [http://www. | + | [http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 '''[3]:'''] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, ''Annual Reviews of Genetics'', '''28''', 117-139. |
Revision as of 11:28, 24 October 2010
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Description
G28 !
THIS PAGE IS CURRENTLY BEING UPDATED.
Performance
Experiment1 | Characteristic1 | Value1 |
---|---|---|
Transfer Function | Maximum Output | 6.6 PoPS cell-1 |
Hill coefficient | 1.6 | |
Switch Point | 1.5E-9 M 3OC6HSL, exogenous | |
Response time: | <1 min | |
Input compatibility | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
Weak response to | C4HSL, C10HSL, C12HSL | |
Stability | Genetic Stability (Low/High Input) |
>92/>56 generations |
Performance Stability (Low/High Input) |
>92/>56 generations | |
Demand | Internal Demand (Low/High Input) |
Not measured |
Transcriptional output demand: (Low/High Input) Nt = length of downstream transcript in nucleotides |
(0/6xNt) nucleotides cell-1 s-1 | |
(0/1.5E-1xNt) RNAP cell-1 | ||
Growth Rate (Low/High Input) |
54/59 min Doubling time |
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3
References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.
[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176.
[http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.