Difference between revisions of "Part:BBa K2560008"
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<a href=" https://www.ncbi.nlm.nih.gov/pubmed/7934914"><abbr title =" de Smit MH, van Duin J., Translational initiation at the coat-protein gene of phage MS2: native upstream RNA relieves inhibition by local secondary structure. (1993) 1079-88" >( Smit MH and Duin J van., 1993)</abbr></a>. | <a href=" https://www.ncbi.nlm.nih.gov/pubmed/7934914"><abbr title =" de Smit MH, van Duin J., Translational initiation at the coat-protein gene of phage MS2: native upstream RNA relieves inhibition by local secondary structure. (1993) 1079-88" >( Smit MH and Duin J van., 1993)</abbr></a>. | ||
The effectiveness of this sequence is determined by its base-pairing potential to the ribosome and its spacing from the start codon | The effectiveness of this sequence is determined by its base-pairing potential to the ribosome and its spacing from the start codon | ||
− | <a href=" http://www.sciencedirect.com/science/article/pii/S0022283605800245"><abbr title =" Maarten H.de | + | <a href=" http://www.sciencedirect.com/science/article/pii/S0022283605800245"><abbr title =" Maarten H. de Smit, Jan van Duin, Translational initiation on structured messengers: Another role for the shine-dalgarno interaction (1994) 173-184" >( Smit MH and Duin J van., 1994)</abbr></a>. The presence of the RBS is crucial for protein translation. |
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Revision as of 10:30, 17 October 2018
Phytobrick version of BBa_B0034
This is the Phytobrick version of the ribosome binding site BBa_B0034 and was build as a part of the Marburg Collection. Instructions of how to use the Marburg Collection are provided at the bottom of the page.
Overview
The ribosome binding site (RBS) is a short sequence upstream the transcription start. It can be identified easily by the typical Shine-Dalgarno sequence that pairs with the anti Shine-Dalgarno sequence at the 3’ end of the 16S rRNA ( Smit MH and Duin J van., 1993). The effectiveness of this sequence is determined by its base-pairing potential to the ribosome and its spacing from the start codon ( Smit MH and Duin J van., 1994). The presence of the RBS is crucial for protein translation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Marburg Toolbox
We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids.
36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk.
The Marburg Collection contains 123 parts in total, including:
inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.