Difference between revisions of "Part:BBa F2620"
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'''Description'''<br> | '''Description'''<br> | ||
| − | A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>''). ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]]. ''Device output'' is [[PoPS]] from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. | + | A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>''). ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]]. ''Device output'' is [[PoPS]] from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet [[:Image:F2620submit.pdf|here]]. |
Revision as of 21:19, 25 August 2007
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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet here.
Performance
| Experiment1 | Characteristic1 | Value1 |
|---|---|---|
| Transfer Function | Input Swing | 1E-9 - 1E-6 M 3OC6HSL, exogenous |
| Output Swing | 0±1 - 503±1 GFP molecules cfu-1 s-1 | |
| Switch Point | 7E-9 ±1E-9 M 3OC6HSL, exogenous | |
| Response time: | Time from 0 - 50% output | 9 min |
| Time from 0 - 90% output | 27 min | |
| Time from 100 - 50% output | # min | |
| Time from 100 - 5% output | # min | |
| Specificity | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
| Weak response to | C4HSL, C10HSL, C12HSL | |
| Stability | Genetic Stability (Low/High Input) |
>92/74 generations |
| Performance Stability (Low/High Input) |
>92/74 generations | |
| Demand | Translation Demand: (Low/High Input) |
5040/141600 charged tRNA cfu-1 s-1 |
| 336/9440 ribosomes sequestered cfu-1 | ||
| Transcription Demand: (Low/High Input) |
(#/#) nucleotides cfu-1 s-1 | |
| (#/#) RNAP sequestered cfu-1 | ||
| Growth Rate (Low/High Input) |
54/59 min doubling time |
1Measured by Ania Labno and Barry Canton 2006-2007
Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.