Difference between revisions of "RFC25 "Freiburg standard" parts"

(→‎Fluorescent proteins)
(→‎Reptorter proteins)
Line 1: Line 1:
−
=Reptorter proteins=
+
=RFC25 BioBrick parts=
 
==Fluorescent proteins==
 
==Fluorescent proteins==
 
{| class="wikitable"
 
{| class="wikitable"
Line 109: Line 109:
 
|-
 
|-
 
|Firefly luciferase
 
|Firefly luciferase
−
|BBa_K525999
+
|BBa_K525999<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_I757009</A></HTML>
 
|61
 
|61
 
|-
 
|-
Line 139: Line 139:
 
|Short Linker
 
|Short Linker
 
|(Gly-Gly-Ser-Gly)
 
|(Gly-Gly-Ser-Gly)
−
|BBa_K243004
+
|BBa_K243004<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_I757009</A></HTML>
 
|4
 
|4
 
|-
 
|-
 
|Middle Linker  
 
|Middle Linker  
 
|2x (Gly-Gly-Ser-Gly)
 
|2x (Gly-Gly-Ser-Gly)
−
|BBa_K243005
+
|BBa_K243005<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_I757009</A></HTML>
 
|8
 
|8
 
|-
 
|-
 
|Long Linker
 
|Long Linker
 
|3x (Gly-Gly-Ser-Gly)
 
|3x (Gly-Gly-Ser-Gly)
−
|BBa_K243006
+
|BBa_K243006<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_I757009</A></HTML>
 
|12
 
|12
 
|-
 
|-
Line 169: Line 169:
 
|-
 
|-
 
|Erythromycin Esterase Type II  
 
|Erythromycin Esterase Type II  
−
|BBa_K1159000
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159000'>BBa_K1159000</A></HTML>
 
|-
 
|-
 
|Laccase
 
|Laccase
−
|BBa_K1159002
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159002'>BBa_K1159002</A></HTML>
 
|-
 
|-
 
|Laccase TVEL5
 
|Laccase TVEL5
−
|BBa_K1615067
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1615067'>BBa_K1615067</A></HTML>
 
|-
 
|-
 
|TEV Protease
 
|TEV Protease
−
|BBa_K1159100
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159100'>BBa_K1159100</A></HTML>
 
|-
 
|-
 
|Thermonuclease
 
|Thermonuclease
−
|BBa_K1159105
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159105'>BBa_K1159105</A></HTML>
 
|-
 
|-
 
|Heroin Esterase
 
|Heroin Esterase
−
|BBa_K1615045
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1615045'>BBa_K1615045</A></HTML>
 
|-
 
|-
 
|CRE recombinase
 
|CRE recombinase
−
|BBa_K1680007
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1680007'>BBa_K1680007</A></HTML>
 
|-
 
|-
 
|Monoamine Oxidase A  
 
|Monoamine Oxidase A  
−
|BBa_K1615022
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1615022'>BBa_K1615022</A></HTML>
 
|-
 
|-
 
|Endo-β-Galactosidase
 
|Endo-β-Galactosidase
−
|BBa_K1483001
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1483001'>BBa_K1483001</A></HTML>
 
|-
 
|-
 
|α-Galactosidase
 
|α-Galactosidase
−
|BBa_K1483002
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1483002'>BBa_K1483002</A></HTML>
 
|-
 
|-
 
|Acetylgalactosamindase
 
|Acetylgalactosamindase
−
|BBa_K1483000
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1483000'>BBa_K1483000</A></HTML>
 
|-
 
|-
 
|2-oxoglutarate oxygenase/decarboxylase
 
|2-oxoglutarate oxygenase/decarboxylase
−
|BBa_K1065000
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1065000'>BBa_K1065000</A></HTML>
 
|-
 
|-
 
|}
 
|}
Line 210: Line 210:
 
|-
 
|-
 
|VP16
 
|VP16
−
|BBa_K1150001
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1150001'>BBa_K1150001</A></HTML>
 
|-
 
|-
 
|}
 
|}
Line 218: Line 218:
 
|-
 
|-
 
|TEV Protease
 
|TEV Protease
−
|BBa_K1159101
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159101'>BBa_K1159101</A></HTML>
−
|BBa_K1159102
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159102'>BBa_K1159102</A></HTML>
 
|-
 
|-
 
|SpyCatcher/SpyTag
 
|SpyCatcher/SpyTag
−
|BBa_K1159200
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159200'>BBa_K1159200</A></HTML>
−
|BBa_K1159201
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159201'>BBa_K1159201</A></HTML>
 
|-
 
|-
 
|}
 
|}
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|-
 
|-
 
|Fluorescein binding Anticalin  
 
|Fluorescein binding Anticalin  
−
|BBa_K1159003
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159003'>BBa_K1159003</A></HTML>
 
|-
 
|-
 
|Digoxigenin binding Anticalin
 
|Digoxigenin binding Anticalin
−
|BBa_K243003
+
|BBa_K243003<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K243003'>BBa_K243003</A></HTML>
 
|-
 
|-
 
|HER2 binding DARPin
 
|HER2 binding DARPin
−
|BBa_K404302
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K404302'>BBa_K404302</A></HTML>
 
|-
 
|-
 
|HER2 binding Affibody
 
|HER2 binding Affibody
−
|BBa_K404302
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_K404302</A></HTML>
 
|-
 
|-
 
|Nickel Binding Protein
 
|Nickel Binding Protein
−
|BBa_K1321009
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1321009'>BBa_K1321009</A></HTML>
 
|-
 
|-
 
|Cellulose binding Domain
 
|Cellulose binding Domain
−
|BBa_K863110
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K863110'>BBa_K863110</A></HTML>
 
|}
 
|}
 
==Affinity tags==
 
==Affinity tags==
Line 253: Line 253:
 
|-
 
|-
 
|HA tag
 
|HA tag
−
|BBa_K1150016
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1150016'>BBa_K1150016</A></HTML>
 
|-
 
|-
 
|His<sub>6</sub>-tag
 
|His<sub>6</sub>-tag
−
|BBa_I757013
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757013'>BBa_I757013</A></HTML>
 
|-
 
|-
 
|SpyTag
 
|SpyTag
−
|BBa_K1159020
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159020'>BBa_K1159020</A></HTML>
 
|-
 
|-
 
|''Strep''-tag II as N-part
 
|''Strep''-tag II as N-part
−
|BBa_K549018
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K549018'>BBa_K549018</A></HTML>
 
|-
 
|-
 
|''Strep''-tag II
 
|''Strep''-tag II
−
|BBa_J18916
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_J18916'>BBa_J18916</A></HTML>
 
|-
 
|-
 
|cMyc-tag
 
|cMyc-tag
−
|BBa_K823036
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K823036'>BBa_K823036</A></HTML>
 
|-
 
|-
 
|3x FLAG tag
 
|3x FLAG tag
−
|BBa_K823034
+
|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K823034'>BBa_K823034</A></HTML>
 
|}
 
|}

Revision as of 00:26, 15 May 2016

RFC25 BioBrick parts

Fluorescent proteins

Fluorescent proteins
Protein Color BioBrick Excitation [nm] Emission [nm] Quantum Yield
Cerulean Cyan BBa_I757009 433 475 0.62
GFP Green BBa_K648013 484 507 0.60
GFP Green BBa_K863120 484 507 0.60
GFP Green BBa_K823039 484 507 0.60
sfGFP Green BBa_K1321337 485 510 0.65
Dronpa (photoactivatable) Green BBa_K1680006 503 518 0.85
mVenus Yellow BBa_I757008 515 528 0.57
sYFP2 Yellow BBa_K1159301 514 527 0.61
eCFP Cyan BBa_K1159302 439 476 0.40
mRFP Red BBa_K1351021 584 607 0.25
mRFP Red BBa_K1615089 584 607 0.25
mKate2 Red BBa_K823029 588 633

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein BioBrick Size [kDa]
Firefly luciferase BBa_K525999BBa_I757009 61
Renilla luciferase 36
NanoLuc luciferase BBa_K1159001 19
Gaussia luciferase

Transmembrane Domains

Linker domains

Linker
Protein Sequence BioBrick Length [residues]
Short Linker (Gly-Gly-Ser-Gly) BBa_K243004BBa_I757009 4
Middle Linker 2x (Gly-Gly-Ser-Gly) BBa_K243005BBa_I757009 8
Long Linker 3x (Gly-Gly-Ser-Gly) BBa_K243006BBa_I757009 12

Catalytic domains

Fluorescent proteins
Erythromycin Esterase Type II BBa_K1159000
Laccase BBa_K1159002
Laccase TVEL5 BBa_K1615067
TEV Protease BBa_K1159100
Thermonuclease BBa_K1159105
Heroin Esterase BBa_K1615045
CRE recombinase BBa_K1680007
Monoamine Oxidase A BBa_K1615022
Endo-β-Galactosidase BBa_K1483001
α-Galactosidase BBa_K1483002
Acetylgalactosamindase BBa_K1483000
2-oxoglutarate oxygenase/decarboxylase BBa_K1065000

Transcription Regulators

Fluorescent proteins
VP16 BBa_K1150001

Split proteins

Fluorescent proteins
TEV Protease BBa_K1159101 BBa_K1159102
SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201

Binding proteins

Fluorescent proteins
Fluorescein binding Anticalin BBa_K1159003
Digoxigenin binding Anticalin BBa_K243003BBa_K243003
HER2 binding DARPin BBa_K404302
HER2 binding Affibody BBa_K404302
Nickel Binding Protein BBa_K1321009
Cellulose binding Domain BBa_K863110

Affinity tags

Affinity tags
HA tag BBa_K1150016
His6-tag BBa_I757013
SpyTag BBa_K1159020
Strep-tag II as N-part BBa_K549018
Strep-tag II BBa_J18916
cMyc-tag BBa_K823036
3x FLAG tag BBa_K823034