Difference between revisions of "Part:BBa F2620"
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[[Plasmids|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br> | [[Plasmids|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br> | ||
[[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'', ''<partinfo>BBa_E0434</partinfo>'', ''<partinfo>BBa_E0240</partinfo>''<br> | [[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'', ''<partinfo>BBa_E0434</partinfo>'', ''<partinfo>BBa_E0240</partinfo>''<br> | ||
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Crosstalk with systems containing ''<partinfo>BBa_C0040</partinfo>''.<br> | Crosstalk with systems containing ''<partinfo>BBa_C0040</partinfo>''.<br> | ||
| + | [[Help:Signalling|''Cell-Cell Signaling Systems:'']]Crosstalk with devices using [[3OC6HSL|3OC<sub>6</sub>HSL]], C6HSL, C7HSL, C8HSL, C10HSL. | ||
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</div> | </div> | ||
Revision as of 21:23, 5 October 2006
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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
| Characteristics | |||
| Input Swing: | 0.1 - 1000 nM 3OC6HSL, exogenous | L/H Response time: | 25 mins |
| Output Swing: | 21±3 - 590±9 GFP molecules cfu-1 s-1 | Genetic Stability: | >92/74 generations (Low/High Input) |
| Switch Point: | 1E-8 M 3OC6HSL, exogenous | Performance Stability: | >92/74 generations (Low/High Input) |
| Transfer Function |
Specificity |
| Response time |
Stability |
| Demand | |||
| Translation Demand: (Low/High Input) |
5040/141600 charged tRNA cfu-1 s-1 336/9440 ribosomes sequestered cfu-1 |
Growth Rate: | 54/59 min doubling time (Low/High Input) |
Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems:Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.