Difference between revisions of "Part:BBa F2620"
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|width='80px'|[[Input Swing|''Input Swing:'']] | |width='80px'|[[Input Swing|''Input Swing:'']] | ||
− | |width='260px'| | + | |width='260px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous |
|width='130px' align='left'|[[Resonse time|''L/H Response time:'']] | |width='130px' align='left'|[[Resonse time|''L/H Response time:'']] | ||
− | |width='210px' align='left'| | + | |width='210px' align='left'|25 mins |
|- | |- | ||
|[[Output Swing|''Output Swing:'']] | |[[Output Swing|''Output Swing:'']] | ||
− | | | + | |21±3 - 590±9 GFP molecules cfu<sup>-1</sup> s<sup>-1</sup> |
|align='left'|[[Genetic Stability|''Genetic Stability:'']] | |align='left'|[[Genetic Stability|''Genetic Stability:'']] | ||
− | | | + | |>92/74 generations (Low/High Input) |
|- | |- | ||
|[[Switch Point|''Switch Point:'']] | |[[Switch Point|''Switch Point:'']] | ||
− | | | + | |1E-8 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous |
|align='left'|[[Performance Stability|''Performance Stability:'']] | |align='left'|[[Performance Stability|''Performance Stability:'']] | ||
− | | | + | |>92/74 generations (Low/High Input) |
|} | |} | ||
{|align="center" cellspacing="0" cellpadding="0" | {|align="center" cellspacing="0" cellpadding="0" | ||
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|width='680px' colspan="4" valign="top"|'''Demand''' | |width='680px' colspan="4" valign="top"|'''Demand''' | ||
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− | |width='125px' valign="top"| [[Translation Demand|''Translation Demand:'']]<br>( | + | |width='125px' valign="top"| [[Translation Demand|''Translation Demand:'']]<br>(Low/High Input) |
− | |width=' | + | |width='235px' valign="top"| 5040/141600 charged tRNA cfu<sup>-1</sup> s<sup>-1</sup><br>336/9440 ribosomes sequestered cfu<sup>-1</sup> |
− | |width=' | + | |width='105px' valign="top"| [[Growth Rate|''Growth Rate:'']] |
|width='215px' valign="top"| 54/59 min doubling time (Low/High Input) | |width='215px' valign="top"| 54/59 min doubling time (Low/High Input) | ||
|} | |} |
Revision as of 21:19, 5 October 2006
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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
Characteristics | |||
Input Swing: | 0.1 - 1000 nM 3OC6HSL, exogenous | L/H Response time: | 25 mins |
Output Swing: | 21±3 - 590±9 GFP molecules cfu-1 s-1 | Genetic Stability: | >92/74 generations (Low/High Input) |
Switch Point: | 1E-8 M 3OC6HSL, exogenous | Performance Stability: | >92/74 generations (Low/High Input) |
Transfer Function |
Specificity |
Response time |
Stability |
Demand | |||
Translation Demand: (Low/High Input) |
5040/141600 charged tRNA cfu-1 s-1 336/9440 ribosomes sequestered cfu-1 |
Growth Rate: | 54/59 min doubling time (Low/High Input) |
Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here.
Crosstalk with systems containing BBa_C0040.