Difference between revisions of "Part:BBa F2620"

Revision as of 19:31, 19 September 2006

A work in progress...

Input:	3OC₆HSL
Output:	PoPS

tetR R0040	LuxR I0462	lux pR R0062

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040. Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator.

Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics

Input Swing:	## nM 3OC₆HSL, exogenous
Output Swing:	#-# PoPS
Switch Point:	2 nM 3OC₆HSL, exogenous
L/H Latency:	# minutes

Key Subparts

BBa_R0040:	TetR-regulated operator
BBa_C0062:	luxR ORF
BBa_R0062:	LuxR-regulated operator

Transfer Function	Specificity
Latency	Stability

Demand

Transcription Demand:	## NTP per second ## polymerases sequestered
Translation Demand:	# charged tRNA per second # ribosomes sequestered

Stability

Genetic Stability:	##/## replication events (Lo Input/High Input)
Performance Stability:	##/## replication events (Lo Input/High Input)

Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.

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-{|
+{{Template:F2620 page header|
-|width="240px"|<span style="font-family: Arial Black; font-size: 18pt">BBa_F2620</span><br>
+|Last Update=15th Sept. 2006
+}}
-<span style="font-family: Arial; font-size: 12pt">3OC<sub>6</sub>HSL -> PoPS Receiver</span><br>
-|width="240px" align="center" valign="top"|[[Image:F2620.jpg|200px]]
-|width="240px" align="center" valign="bottom"|<html><DIV><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
-promoter (tetR, negative)' src='/r/parts/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
-luxR Protein Generator' src='/r/parts/images/partbypart/icon_generator.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=192'><img title='BBa_R0062
-Promoter (luxR &amp; HSL regulated -- lux pR)' src='/r/parts/images/partbypart/icon_regulatory.png' height='25' border=0></A></TABLE></DIV></html>
-|}
-<span style="align: left>
-Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Last Update: Sept. 15th, 2006
-</span>
-----
 <div style="padding: 00px; width: 720px; border: 0px solid #000000;">