Difference between revisions of "Plasmid backbones/Measurement"

Revision as of 13:14, 20 February 2009


Part assembly	System operation	Protein expression	Assembly of protein fusions	Part measurement	Screening of part libraries	Building BioBrick vectors	DNA synthesis	Other standards	Archive

Or get some help on plasmid backbones.

Some plasmids are designed to facilitate the measurement of different part classes:

Promoters

The property of promoters that should be measured to best support the reuse of these parts is the promoter clearance rate or promoter activity. The promoter activity is defined as the number of RNA polymerase molecules that pass by (or clear) the final base pair of the promoter and continue along the DNA as an elongation complex. Promoter activity can be characterized by the number of polymerases exiting the terminal base pair of the promoter (in units of Polymerases per Second, or [http://www.nature.com/nature/comics/syntheticbiologycomic/ PoPS]). PoPS is difficult to measure directly and to date Registry users have characterized promoters in relative or ratio units.

Part:BBa_J23101 - A collection of promoters characterized by Chris Anderson. The plasmid backbone from these parts can be used to measure the activity of other promoters based on expression of RFP.
There is a kit of components available that is described here to measure promoter activity in a shared, standard unit: Relative Promoter Units (RPUs). The kit makes use of pSB3K3 as the plasmid and BBa_E0240 (GFP) to measure promoter activity.

Inverters

Part:pSB1A10 - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of inverter devices.

References

Endy05 pmid=

</biblio>

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 ===Promoters===
-The property of promoters that best supports the reuse of these parts is the promoter clearance rate or promoter activity.  The promoter activity is defined as the number of RNA polymerase molecules that pass by (or clear) the final base pair of the promoter and continue along the DNA as an elongation complex.  Promoter activity can be characterized by the number of polymerases exiting the terminal base pair of the promoter (in units of Polymerases per Second, or [http://www.nature.com/nature/comics/syntheticbiologycomic/ PoPS]).  PoPS is difficult to measure directly and to date Registry users have characterized promoters in relative or ratio units.
+The property of promoters that should be measured to best support the reuse of these parts is the promoter clearance rate or promoter activity.  The promoter activity is defined as the number of RNA polymerase molecules that pass by (or clear) the final base pair of the promoter and continue along the DNA as an elongation complex.  Promoter activity can be characterized by the number of polymerases exiting the terminal base pair of the promoter (in units of Polymerases per Second, or [http://www.nature.com/nature/comics/syntheticbiologycomic/ PoPS]).  PoPS is difficult to measure directly and to date Registry users have characterized promoters in relative or ratio units.
 *[[Part:BBa_J23101]] - A collection of promoters characterized by Chris Anderson.  The plasmid backbone from these parts can be used to measure the activity of other promoters based on expression of RFP.
 *There is a kit of components available that is described [[Measurement|here]] to measure promoter activity in a shared, standard unit: Relative Promoter Units (RPUs).  The kit makes use of [[Part:pSB3K3|pSB3K3]] as the plasmid and [[Part:BBa_E0240|BBa_E0240]] (GFP) to measure promoter activity.