Difference between revisions of "Help:Terminators"
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*[[Terminators/Design|'''How to design a terminator''']] | *[[Terminators/Design|'''How to design a terminator''']] | ||
*[[Help:Terminators/Construction|'''How to construct an terminator''']] | *[[Help:Terminators/Construction|'''How to construct an terminator''']] | ||
+ | *[[Help:Terminators/Measurement|'''How to measure a terminator's termination efficiency''']] | ||
*[[Help:Terminators/Further reading|'''Further reading''']] about terminator sequence and function | *[[Help:Terminators/Further reading|'''Further reading''']] about terminator sequence and function |
Revision as of 22:11, 28 September 2008
Terminators are genetic parts that usually occur at the end of a gene or operon and cause transcription to stop. In prokaryotes, terminators usually fall into two categories (1) rho-independent terminators and (2) rho-dependent terminators.
Rho-independent terminators are generally composed of palindromic sequence that forms a stem loop rich in G-C base pairs followed by several T bases. The conventional model of transcriptional termination is that the stem loop causes RNA polymerase to pause and transcription of the poly-A tail causes the RNA:DNA duplex to unwind and dissociate from RNA polymerase.
All the E. coli terminators in the Registry are rho-independent terminators. Rho-dependent terminators are not included, because rho-dependent terminators are not specified by sequence.
- How do terminators work?
- Glossary of terms relating to terminators
- Browse the terminator parts available from the registry
- How to design a terminator
- How to construct an terminator
- How to measure a terminator's termination efficiency
- Further reading about terminator sequence and function