Difference between revisions of "Part:BBa F2620"

Revision as of 03:41, 8 July 2008

Input:	3OC₆HSL
Output:	PoPS

tetR R0040	LuxR I0462	lux pR R0062

Part Main Page Transfer Function Specificity Response time Stability Add Data

Description
A transcription factor, LuxR (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet here.

Performance

Experiment¹	Characteristic¹	Value¹
Transfer Function	Maximum Output	6.6 PoPS cell^-1
	Hill coefficient	1.6
	Switch Point	1.5E-9 M 3OC₆HSL, exogenous
Response time:	<1 min
Input compatibility	Strong response to	3OC₆HSL, C₆HSL , C₇HSL, 3OC₈HSL, C₈HSL
Input compatibility	Weak response to	C₄HSL, C₁₀HSL, C₁₂HSL
Stability	Genetic Stability (Low/High Input)	>92/>56 generations
Stability	Performance Stability (Low/High Input)	>92/>56 generations
Demand	Internal Demand (Low/High Input)	Not measured
	Transcriptional output demand: (Low/High Input)	(nd/6xNt) nucleotides cell^-1 s^-1
	Transcriptional output demand: (Low/High Input)	(nd/1.5E-1xNt) RNAP cell^-1
	Growth Rate (Low/High Input)	54/59 min Doubling time

¹Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC₆HSL, C6HSL, C7HSL, C8HSL, C10HSL.

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 '''Description'''<br>
-A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.  Get the pdf datasheet  [[:Image:F2620DataSheetV5.pdf|here]].
+A transcription factor, LuxR (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a TetR (''<partinfo>BBa_C0040</partinfo>'') regulated operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.  Get the pdf datasheet  [[:Image:F2620DataSheetV5.pdf|here]].