Difference between revisions of "Part:BBa F2620"
m |
|||
| Line 16: | Line 16: | ||
|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']] | |rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']] | ||
|''Maximum Output'' | |''Maximum Output'' | ||
| − | |6. | + | |6.6 [[PoPS]] cell<sup>-1</sup> |
|- | |- | ||
|''Hill coefficient'' | |''Hill coefficient'' | ||
| − | |1. | + | |1.6 |
|- | |- | ||
|[[Switch Point|''Switch Point'']] | |[[Switch Point|''Switch Point'']] | ||
| − | | | + | |1.5E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous |
|- | |- | ||
|[[Part:BBa_F2620:Response time|'''Response time:''']] | |[[Part:BBa_F2620:Response time|'''Response time:''']] | ||
| Line 45: | Line 45: | ||
|Not measured | |Not measured | ||
|- | |- | ||
| − | |rowspan="2"|[[Transcription Demand|'' | + | |rowspan="2"|[[Transcription Demand|''Transcriptional output demand:'']]<br>(Low/High Input) |
|(nd/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup> | |(nd/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup> | ||
|- | |- | ||
Revision as of 15:36, 11 February 2008
|
|
|
Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet here.
Performance
| Experiment1 | Characteristic1 | Value1 |
|---|---|---|
| Transfer Function | Maximum Output | 6.6 PoPS cell-1 |
| Hill coefficient | 1.6 | |
| Switch Point | 1.5E-9 M 3OC6HSL, exogenous | |
| Response time: | <1 min | |
| Input compatibility | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
| Weak response to | C4HSL, C10HSL, C12HSL | |
| Stability | Genetic Stability (Low/High Input) |
>92/>56 generations |
| Performance Stability (Low/High Input) |
>92/>56 generations | |
| Demand | Internal Demand (Low/High Input) |
Not measured |
| Transcriptional output demand: (Low/High Input) |
(nd/6xNt) nucleotides cell-1 s-1 | |
| (nd/1.5E-1xNt) RNAP cell-1 | ||
| Growth Rate (Low/High Input) |
54/59 min Doubling time |
1Measured by Ania Labno and Barry Canton 2006-2007
Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.